LABORATORY REPORT (Click on the Save a reproduction get-up-and-go on the panel above to save your report) drill: realise: Instructor: Date: Enzyme Activity Predictions 1. sucrase post have the sterling(prenominal) activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing saccharose concentration. Materials and Methods middle of pH on Enzyme Activity. 1. myrmecophilous Variable. gist of product (glucose and fructose) produced 2. self-governing Variable. pH 3. Controlled Variables. temperature; amount of substrate ( sucrose) show; sucrase + sucrose brooding time arrange of Temperature on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. indie Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) generate; sucrase + sucrose incubation time issuance of substratum Concentration on Enzyme Activity. 1. Dependent Varia ble. amount of product (glucose and fructose) produced 2. Independent Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time 1. name what is measured as an indicant of sucrase activity and why this is an indicator of sucrase activity. indefinite 2. inform why modify sucrase was used as a control. undefined 3. Alkaline DNS denatures sucrase. Explain why it is all important(p) to denature the enzyme. undefined Results sidestep 1: military unit of pH on Sucrase Activity 1 2 3 average ph 3 0.1702 0.1758 0.1504 0.165 ph 4 0.334 0.296 0.303 0.311 ph 5 0.664 0.657 0.645 0.655 Optical Density ph 6 0.921 0.929 0.954 0.935 ph 7 0.793 0.819 0.776 0.796 ph 8 0.43 0.397 0.429 0.419 ph 9 0.169 0.195 0.171 0.178 Effect of pH on Sucrase Activity 1. State the optimal pH for sucrase activity and how sucrase activity changes at more than acidic and alkaline pH values. undefined Table 2: Effect of Temperatur e on Sucrase Activity 10 °C (50 °F) 0.008! 0.005 0.007 0.007 20 °C (68 °F) 0.277...If you want to get a full essay, order it on our website: BestEssayCheap.com
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